空肠弯曲杆菌
糖复合物
生物膜
微生物学
凝集素
聚糖
硫黄素
岩藻糖
生物化学
生物
血凝素(流感)
甘露糖
唾液酸
化学
半乳糖
糖蛋白
细菌
遗传学
疾病
阿尔茨海默病
医学
病理
基因
作者
Hana Turoňová,Thomas R. Neu,Pavel Ulbrich,J. Pazlarová,Odile Tresse
出处
期刊:Biofouling
[Informa]
日期:2016-04-20
卷期号:32 (5): 597-608
被引量:20
标识
DOI:10.1080/08927014.2016.1169402
摘要
Campylobacter jejuni is responsible for the most common bacterial foodborne gastroenteritis. Despite its fastidious growth, it can survive harsh conditions through biofilm formation. In this work, fluorescence lectin-binding analysis was used to determine the glycoconjugates present in the biofilm matrix of two well-described strains. Screening of 72 lectins revealed strain-specific patterns with six lectins interacting with the biofilm matrix of both strains. The most common sugar moiety contained galactose and N-acetylgalactosamine. Several lectins interacted with N-acetylglucosamine and sialic acid, probably originated from the capsular polysaccharides, lipooligosaccharides and N-glycans of C. jejuni. In addition, glycoconjugates containing mannose and fucose were detected within the biofilm, which have not previously been found in the C. jejuni envelope. Detection of thioflavin T and curcumin highlighted the presence of amyloids in the cell envelope without association with specific cell appendages. The lectins ECA, GS-I, HMA and LEA constitute a reliable cocktail to detect the biofilm matrix of C. jejuni.
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