Orthogonal experimental study on preparation of chitosan oligosaccharide based on cellulase hydrolysis

Abstract The conditions of enzymatic hydrolysis of chitosan by cellulase were studied by orthogonal experiments. The effects of temperature, pH, reaction time and enzyme dosage on the decomposition efficiency of chitosan were investigated and the following conclusions were obtained by experiments: the major and minor relationship between the factors affecting the conversion of chitosan oligosaccharide prepared by cellulase hydrolysis is temperature > pH > time. The optimum conditions are as follows: at the reaction temperature of 50 °C, the pH of 4.8 and the hydrolysis time of 3.5 h. Under these reaction conditions, the content of reducing sugar is the highest, which is 1.59 mg⋅mL -1 , and the economic benefit is the best.