基因组编辑
里氏木霉
清脆的
Cas9
基因组
转化(遗传学)
突变
计算生物学
生物
基因
遗传学
突变
生物化学
纤维素酶
纤维素
出处
期刊:Methods in molecular biology
日期:2020-11-10
卷期号:: 87-98
被引量:8
标识
DOI:10.1007/978-1-0716-1048-0_8
摘要
In this protocol, we describe the establishment of a CRISPR/Cas9 system in Trichoderma reesei by generating a specific, codon-optimized Cas9-expressing strain and by in vitro transcription of a gRNA. This system induces mutagenesis or introduces a gene in a targeted way based on PEG-mediated protoplast transformation. Up to three targets, multiplexed genome editing can be obtained in one transformation.
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