适体
核酸外切酶 III
卡那霉素
检出限
核酸外切酶
鱼精蛋白
互补DNA
化学
DNA
生物传感器
选择性
组合化学
生物物理学
色谱法
分子生物学
生物化学
生物
DNA聚合酶
基因
肝素
大肠杆菌
催化作用
作者
Jingwen Li,Yongming Liu,Lin Hao,Yan Chen,Zhenbo Liu,Xuming Zhuang,Chunyuan Tian,Xiuli Fu,Lingxin Chen
出处
期刊:Food Chemistry
[Elsevier]
日期:2021-06-01
卷期号:347: 128988-128988
被引量:25
标识
DOI:10.1016/j.foodchem.2020.128988
摘要
A label-free colorimetric method based on exonuclease I (Exo I)-assisted signal amplification with protamine as a medium was developed for analysis of kanamycin. In this study, a double-stranded DNA (dsDNA) probe was tailored by manipulating an aptamer and its complementary DNA (cDNA) ensuring detection of target with high selectivity and excellent sensitivity. Herein, protamine could not only combine with negatively charged gold nanoparticles but also interaction with polyanion DNA. Upon addition of target kanamycin, the target-aptamer complex was formed and the cDNA was released. Thus, both aptamer and cDNA could be digested by Exo I, and the captured kanamycin was liberated for triggering target recycling and signal amplification. Under optimized conditions, the proposed colorimetric method realized a low detection limit of 2.8 × 10−14 M along with a wide linear range plus excellent selectivity. Our strategy exhibited enormous potentials for fabricate various kinds of biosensors based on target-induced aptamer configuration changes.
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