烟曲霉
毕赤酵母
突变体
重组DNA
酶动力学
毕赤酵母
生物化学
葡萄糖苷酶
化学
酿酒酵母
真菌蛋白
生物
微生物学
酶
基因
活动站点
作者
Sidrah Nazir,Muhammad Asad,Syed Muhammad Saqlan Naqvi,Tayyaba Zainab,Saad Imran Malik,Raja Tahir Mehmood,Jehangir Khan,Tasawar Sultana,Nazim Nasir,Atiq Hassan
出处
期刊:Bioresources
[BioResources]
日期:2022-05-14
卷期号:17 (2): 3057-3066
标识
DOI:10.15376/biores.17.2.3057-3066
摘要
β-glucosidases (3.2.1.21) are present in all domains of living organisms, and their importance in a number of essential biological processes and industrial applications has been highlighted. They are interesting for biomass conversion because b-glucans are the world’s largest source of biomass. For this reason, several fungal β-glucosidases have been investigated. The β-glucosidase gene of Aspergillus fumigatus, as well as its mutants D262E and W263F, were cloned and expressed in Pichia pastoris in this study. Their optimum temperature, pH, glucose tolerance, metal ion effect, and Vmax, km, and kcat were determined. The optimal temperature for recombinant β-glucosidase was 65 °C. For mutant D262E, there is an improvement in pH stability ranging from 4 to 6. As compared to the D262E mutant and recombinant β-glucosidase, mutant W263F showed a higher glucose tolerance and kcat.
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