前病毒
生物
祖细胞
病毒学
细胞培养
神经干细胞
星形胶质细胞
小胶质细胞
干细胞
免疫学
细胞生物学
神经科学
中枢神经系统
遗传学
基因
炎症
基因组
作者
Amélie Bauer,Ruth Brack‐Werner
出处
期刊:Methods in molecular biology
日期:2022-01-01
卷期号:: 103-114
被引量:3
标识
DOI:10.1007/978-1-0716-1871-4_10
摘要
Neurocognitive disorders continue to occur in HIV-infected individuals, despite successful antiretroviral therapy. HIV can persist in the brain for decades, where it infects mainly microglial cells and astrocytes. Brain tissues from HIV-infected individuals have been shown to harbor HIV proviruses and to express early viral products with neurotoxic properties, like Tat. Egress of HIV from astrocytes to the periphery in animals further supports a critical role of astrocytes as HIV reservoirs. In vitro studies show that astrocytes can harbor latent HIV proviruses that can be activated by various agents and initiate productive infection of immune cells. Cell culture studies of HIV-infection of astrocytes have depended heavily on rapidly dividing cells derived from tumors or from fetal tissue. However, in adult brains the majority of astrocytes are nondividing. Therefore, cell culture models are needed to investigate the unique properties of latent HIV proviruses in differentiated astrocytes and to compare these with the properties of other HIV reservoirs.This protocol gives guidelines for the culture of the human neural stem cell line HNSC.100 and a stable subpopulation with latent HIV-1 provirus, HNSCLatGFP1.2. The HNSC.100 cell line provides a single cell model system for the study of HIV persistence in proliferating progenitor cells as well as fully differentiated, nondividing astrocytes. The HNSCLatGFP1.2 cell line contains a full-length HIV-1 provirus derived from NL4-3 with GFP-coding sequences in a defective Env reading frame, enabling handling under Biosafety level 2 conditions and convenient observation of provirus reactivation by monitoring GFP expression. The latent provirus can be reactivated by latency reversing agents which allows the analysis of novel latency reversing agents as well as inhibitors of reactivators of latency.
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