骨髓
谱系(遗传)
细胞生物学
舱室(船)
前体细胞
细胞
髓腔
病理
流式细胞术
免疫分型
免疫学
生物
B细胞
解剖
医学
遗传学
抗体
基因
地质学
海洋学
作者
Amélie Bonaud,Karl Balabanian,Marion Espéli
出处
期刊:Methods in molecular biology
日期:2021-01-01
卷期号:: 95-105
被引量:2
标识
DOI:10.1007/978-1-0716-1425-9_8
摘要
B cell development is a stepwise process encompassing several B cell precursor stages that can be phenotypically distinguished, and that is taking place in the bone marrow in adults. Interestingly, within the bone marrow B cell precursors coexist with the most differentiated actors of this lineage, the plasma cells. In this chapter, we describe a method to recover cells from the bone marrow and a flow cytometric approach to identify each subpopulation of the B cell lineage that resides within the bone marrow compartment. This protocol focuses on membrane markers to discriminate all the B cell subpopulations in order to preserve cell integrity during experimentation and for further analyses.
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