Enzyme-free ratiometric fluorescence and colorimetric dual-signal determination of glyphosate based on copper nanoclusters (ZIF/CuNCs) combined with blue carbon dots (bCDs)

A novel ratio fluorescent and colorimetric dual-signal sensing platform for detecting glyphosate based on blue carbon dots (bCDs) combined with ZIF/CuNCs nanomaterials that encapsulate copper nanoclusters (CuNCs) in a metal-organic framework (MOF). In principle, the immobilization of Cu2+ in ZIF/CuNCs results in complexation with imidazole in ZIF, leading to fluorescence quenching of ZIF/CuNCs, while the reference fluorophore bCDs remains unaffected. In addition, the colorimetric sensing strategy was based on the efficient peroxidase-like activity of bCDs binding to Cu2+, catalyzing H2O2 to generate OH. Under this condition, TMB could be oxidized to form blue oxTMB. However, when glyphosate was involved in the system, the fluorescence of ZIF/CuNCs was restored upon due to the strong chelation between Cu2+ and glyphosate, while the peroxidase-like activity of bCDs/Cu2+ decreased and resulted in the generation of fewer oxTMB, accompanied by a lighter blue color. The sensing platform was successfully applied to the determination of glyphosate in real samples of lake water and cabbage, demonstrating reliable and sensitive performance in practical applications.