精原细胞瘤
转录组
生物
肿瘤微环境
癌变
计算生物学
睾丸生殖细胞瘤
生殖细胞
癌症研究
基因表达谱
生物信息学
基因
基因表达
遗传学
肿瘤细胞
化疗
作者
Xin-Jiang Lu,Yanwei Luo,Xiaolu Nie,Bailing Zhang,Xiaoyan Wang,Ran Li,Guangmin Liu,Qianyin Zhou,Zhizhong Liu,Liqing Fan,James M. Hotaling,Zhe Zhang,Hao Bo,Jingtao Guo
标识
DOI:10.1038/s41467-023-44305-9
摘要
Abstract Seminoma is the most common malignant solid tumor in 14 to 44 year-old men. However, its molecular features and tumor microenvironment (TME) is largely unexplored. Here, we perform a series of studies via genomics profiling (single cell multi-omics and spatial transcriptomics) and functional examination using seminoma samples and a seminoma cell line. We identify key gene expression programs share between seminoma and primordial germ cells, and further characterize the functions of TFAP2C in promoting tumor invasion and migration. We also identify 15 immune cell subtypes in TME, and find that subtypes with exhaustion features were located closer to the tumor region through combined spatial transcriptome analysis. Furthermore, we identify key pathways and genes that may facilitate seminoma disseminating beyond the seminiferous tubules. These findings advance our knowledge of seminoma tumorigenesis and produce a multi-omics atlas of in situ human seminoma microenvironment, which could help discover potential therapy targets for seminoma.
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