生物
组蛋白
染色质
突变体
癌变
染色质重塑
细胞生物学
突变
突变
造血
癌症研究
基因
遗传学
干细胞
作者
Yiman Liu,Qinglan Li,Qinglan Li,Lele Song,Chujie Gong,Sylvia Tang,Sylvia Tang,Krista A. Budinich,Ashley Vanderbeck,Kaeli M. Mathias,Gerald Wertheim,Son C. Nguyen,Riley Outen,Eric F. Joyce,Ivan Maillard,Liling Wan
出处
期刊:Cancer Discovery
[American Association for Cancer Research]
日期:2024-04-24
标识
DOI:10.1158/2159-8290.cd-23-0876
摘要
Abstract Gain-of-function mutations in the histone acetylation ‘reader’ ENL, found in AML and Wilms tumor, are known to drive condensate formation and gene activation in cellular systems. However, their role in tumorigenesis remains unclear. Using a conditional knock-in mouse model, we show that mutant ENL perturbs normal hematopoiesis, induces aberrant expansion of myeloid progenitors, and triggers rapid onset of aggressive AML. Mutant ENL alters developmental and inflammatory gene programs in part by remodeling histone modifications. Mutant ENL forms condensates in hematopoietic stem/progenitor cells at key leukemogenic genes, and disrupting condensate formation via mutagenesis impairs its chromatin and oncogenic function. Moreover, treatment with an acetyl-binding inhibitor of mutant ENL displaces these condensates from target loci, inhibits mutant ENL-induced chromatin changes, and delays AML initiation and progression in vivo. Our study elucidates the function of ENL mutations in chromatin regulation and tumorigenesis, and demonstrates the potential of targeting pathogenic condensates in cancer treatment.
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