实时聚合酶链反应
塔克曼
生物
计算生物学
定量分析(化学)
底漆二聚体
分子生物学
聚合酶链反应
数字聚合酶链反应
基因
色谱法
遗传学
化学
多重聚合酶链反应
作者
Chris Heid,Junko Stevens,Kenneth J. Livak,P. Williams
出处
期刊:Genome Research
[Cold Spring Harbor Laboratory]
日期:1996-10-01
卷期号:6 (10): 986-994
被引量:5646
摘要
We have developed a novel "real time" quantitative PCR method. The method measures PCR product accumulation through a dual-labeled fluorogenic probe (i.e., TaqMan Probe). This method provides very accurate and reproducible quantitation of gene copies. Unlike other quantitative PCR methods, real-time PCR does not require post-PCR sample handling, preventing potential PCR product carry-over contamination and resulting in much faster and higher throughput assays. The real-time PCR method has a very large dynamic range of starting target molecule determination (at least five orders of magnitude). Real-time quantitative PCR is extremely accurate and less labor-intensive than current quantitative PCR methods.
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