Interrelationship among protein structure, protein oxidation, lipid oxidation and quality of grass carp surimi during multiple freeze–thaw cycles with different pork backfat contents

Storing at -18°C, the surimi was processed from fresh grass carp, supplemented with pork backfat of 50, 100, or 150 g kg^-1 (groups F₀ , F₅₀ , F₁₀₀ , and F₁₅₀ , respectively). The surimi was thawed and refrozen weekly afterward, with changes in surimi protein structure (primary structure, secondary structure, and tertiary structure), protein oxidation index (carbonyl and sulfhydryl), lipid oxidation index (TBARS), protein solubility, as well as surimi quality (texture characteristics and whiteness), were determined. The results showed that the texture characteristics of surimi increased after the first freezing and thawing (F-T) cycle and then decreased. The whiteness of the surimi decreased with the increase of F-T cycle times, while the whiteness of the surimi increased with the increase in fat content. With the F-T cycle times and fat content rising, the relative content of α-helix structures, sulfhydryl content, and protein solubility decreased. In contrast, carbonyl content was in reverse, being an increasing trend. The free amino content showed a decrease after an increase at the early stage with the F-T cycles growing, while it showed a decrease with an increase in fat content. Meanwhile, the redshift in the maximum absorption peak of the tryptophan fluorescence was caused by the F-T cycles, so was the decline in fluorescence intensity; however, there was little difference in tryptophan spectra with the same fat content. The association among protein structure, protein oxidation, lipid oxidation and surimi quality were elucidated by Pearson's two-tailed correlation. The lipid oxidation in the repeated freezing and thawing process of surimi led to the decrease in whiteness. The protein oxidation led to the change of protein structure and the decrease of protein solubility. Lipid oxidation caused by the increase of fat content led to the decrease of free amino content and protein solubility, and finally led to the increase of whiteness and the deterioration of texture characteristics. PRACTICAL APPLICATIONS: In this study, changes in various components of surimi were measured to examine the interrelationship among protein structure, protein oxidation, lipid oxidation and surimi quality. Freeze-thaw cycle and high-fat content are easy to cause lipid oxidation, protein oxidation, a decrease in protein solubility, and deterioration of surimi quality. Therefore, temperature fluctuations must be avoided to extend the shelf life of such products. An appropriate fat content level must be selected to prevent protein and lipid oxidation and maintain quality.