化学
费斯特共振能量转移
荧光团
半胱氨酸
部分
荧光
光化学
吩嗪
谷胱甘肽
胱胺
共价键
连接器
组合化学
立体化学
有机化学
生物化学
物理
量子力学
酶
计算机科学
操作系统
作者
Lin Yang,Weisong Qu,Xiao Zhang,Yandi Hang,Jianli Hua
出处
期刊:Analyst
[The Royal Society of Chemistry]
日期:2015-01-01
卷期号:140 (1): 182-189
被引量:17
摘要
A molecular chemodosimeter , with a naphthalimide fluorophore connected to a newly designed phenazine energy acceptor, for the selective detection of cysteine was effectively synthesized. featured efficient intramolecular fluorescence resonance energy transfer (FRET) based on spectral overlap between the emission of naphthalimide and the absorption of phenazine. A cystamine chain with a S-S bond was applied to play the role of recognition moiety and the linker part. The specific reaction between the biological thiols and gave rise to an obvious fluorescence intensity enhancement at 540 nm, which is induced by cleavage of the disulfide bond followed by elimination of the FRET effect. High sensitivity and selectivity for cysteine over homocysteine and glutathione were also achieved. In addition, upon excitation at 400 nm, a relatively weak NIR emission provided an internal standard making a promising ratiometric sensor for cellular detection of cysteine.
科研通智能强力驱动
Strongly Powered by AbleSci AI