微管
生物
细胞生物学
微管相关蛋白
磷酸化
高磷酸化
激酶
微管蛋白
基因亚型
微管形核
蛋白激酶A
主轴装置
基因
驱动蛋白
有丝分裂
中心体
诺可达唑
细胞周期蛋白依赖激酶1
微管聚合
遗传学
作者
Gerard Drewes,Andreas Ebneth,Ute Preuss,Eckhard Mandelkow
出处
期刊:Cell
[Elsevier]
日期:1997-04-18
卷期号:89 (2): 297-308
被引量:758
标识
DOI:10.1016/s0092-8674(00)80208-1
摘要
MARK phosphorylates the microtubule-associated proteins tau, MAP2, and MAP4 on their microtubule-binding domain, causing their dissociation from microtubules and increased microtubule dynamics. We describe the molecular cloning, distribution, activation mechanism, and overexpression of two MARK proteins from rat that arise from distinct genes. They encode Ser/Thr kinases of 88 and 81 kDa, respectively, and show similarity to the yeast kin1+ and C. elegans par-1 genes that are involved in the establishment of cell polarity. Expression of both isoforms is ubiquitous, and homologous genes are present in humans. Catalytic activity depends on phosphorylation of two residues in subdomain VIII. Overexpression of MARK in cells leads to hyperphosphorylation of MAPs on KXGS motifs and to disruption of the microtubule array, resulting in morphological changes and cell death.
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