免疫组织化学
结直肠癌
医学
外科肿瘤学
肿瘤科
单变量分析
阶段(地层学)
内科学
临床意义
错义突变
癌症
病理
癌症研究
生物
基因
多元分析
遗传学
突变
古生物学
作者
Kyoung Min Kim,Ae-Ri Ahn,Ho Sung Park,Kyu Yun Jang,Woo Sung Moon,Myoung Jae Kang,Gi Won Ha,Min Ro Lee,Myoung Ja Chung
出处
期刊:BMC Cancer
[Springer Nature]
日期:2022-08-31
卷期号:22 (1)
被引量:15
标识
DOI:10.1186/s12885-022-10039-y
摘要
Abstract In human colorectal cancer (CRC), TP53 is one of the most important driver genes. Immunohistochemistry (IHC) has been used most often to assess the variational status of TP53 . Recently, next-generation sequencing (NGS) of the TP53 gene has increased. However, to our knowledge, a comparison between TP53 status evaluated by IHC and NGS has not been studied. Therefore, the primary aim of this study was to compare the clinical effect of TP53 status evaluated by IHC and NGS in patients with CRC. The secondary aim was to investigate the correlation between expression of p53 by IHC and variational status of TP53 by NGS. We performed immunohistochemical staining of p53 and sequencing of TP53 by NGS in 204 human samples of CRC. We then analyzed the correlation between variational status of TP53 and p53 expression, along with their prognostic impact in CRC patients. There was significant correlation between p53 expression and TP53 variation, TP53 variation and higher N stage, and positive p53 expression and higher N stage. Positive IHC expression of p53 was significantly associated with overall survival (OS) of CRC patients by univariate analysis and was revealed as an independent prognostic factor by multivariate analysis. Additionally, the nonsense/frameshift p53 expression pattern showed a significantly better prognosis than the wild type and missense p53 expression patterns. However, the variational status of TP53 was not significant in OS of CRC patients. These results suggest that IHC expression of p53 protein correlates with variation status of TP53 and expression of p53 protein rather than variation status of TP53 has more significant impact on the OS of CRC patients.
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