自噬
自噬相关蛋白13
生物
细胞生物学
液泡
袋3
蛋白质降解
自噬体
拟南芥
拟南芥
ATG8型
溶酶体
蛋白质水解
生物化学
磷酸化
蛋白激酶A
突变体
蛋白质磷酸化
基因
细胞质
酶
细胞凋亡
作者
Qi Hua,Le Xue,Yao Wang,Shunli Yu,Ting Liu,Shunkang Zhou,Jinyu Chen,Qinfang Chen,Rongliang Qiu,Liwen Jiang,Shi Xiao
出处
期刊:The Plant Cell
[Oxford University Press]
日期:2022-09-02
卷期号:34 (12): 4857-4876
被引量:6
标识
DOI:10.1093/plcell/koac273
摘要
Abstract In multicellular eukaryotes, autophagy is a conserved process that delivers cellular components to the vacuole or lysosome for recycling during development and stress responses. Induction of autophagy activates AUTOPHAGY-RELATED PROTEIN 1 (ATG1) and ATG13 to form a protein kinase complex that initiates autophagosome formation. However, the detailed molecular mechanism underlying the regulation of this protein complex in plants remains unclear. Here, we determined that in Arabidopsis thaliana, the regulatory proteins 14-3-3λ and 14-3-3κ redundantly modulate autophagy dynamics by facilitating SEVEN IN ABSENTIA OF ARABIDOPSIS THALIANA (SINAT)-mediated proteolysis of ATG13a and ATG13b. 14-3-3λ and 14-3-3κ directly interacted with SINATs and ATG13a/b in vitro and in vivo. Compared to wild-type (WT), the 14-3-3λ 14-3-3κ double mutant showed increased tolerance to nutrient starvation, delayed leaf senescence, and enhanced starvation-induced autophagic vesicles. Moreover, 14-3-3s were required for SINAT1-mediated ubiquitination and degradation of ATG13a. Consistent with their roles in ATG degradation, the 14-3-3λ 14-3-3κ double mutant accumulated higher levels of ATG1a/b/c and ATG13a/b than the WT upon nutrient deprivation. Furthermore, the specific association of 14-3-3s with phosphorylated ATG13a was crucial for ATG13a stability and formation of the ATG1–ATG13 complex. Thus, our findings demonstrate that 14-3-3λ and 14-3-3κ function as molecular adaptors to regulate autophagy by modulating the homeostasis of phosphorylated ATG13.
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