圈套复合体
蒙克-18
胞吐
细胞生物学
突触融合蛋白
分泌物
STX1A型
化学
激活剂(遗传学)
膜泡运输蛋白质类
小泡
生物
内体
突触小泡
受体
生物化学
膜
液泡蛋白分选
细胞内
作者
Hao Zhou,Ziqing Wei,Shen Wang,Deqiang Yao,Rongguang Zhang,Cong Ma
出处
期刊:Cell Reports
[Elsevier]
日期:2019-03-01
卷期号:26 (12): 3347-3359.e6
被引量:10
标识
DOI:10.1016/j.celrep.2019.02.064
摘要
Exocytosis of synaptic vesicles and dense-core vesicles requires both the Munc13 and CAPS (Ca2+-dependent activator proteins for secretion) proteins. CAPS contains a soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE)-binding region (called the DAMH domain), which has been found to be essential for SNARE-mediated exocytosis. Here we report a crystal structure of the CAPS-1 DAMH domain at 2.9-Å resolution and reveal a dual role of CAPS-1 in SNARE complex formation. CAPS-1 plays an inhibitory role dependent on binding of the DAMH domain to the MUN domain of Munc13-1, which hinders the ability of Munc13 to catalyze opening of syntaxin-1, inhibiting SNARE complex formation, and a chaperone role dependent on interaction of the DAMH domain with the syntaxin-1/SNAP-25 complex, which stabilizes the open conformation of Syx1, facilitating SNARE complex formation. Our results suggest that CAPS-1 facilitates SNARE complex formation via the DAMH domain in a manner dependent on sequential and cooperative interaction with Munc13-1 and SNARE proteins.
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