线粒体生物发生
骨骼肌
耐力训练
内科学
内分泌学
线粒体
生物发生
氧化磷酸化
β氧化
腓肠肌
安普克
生物
化学
生物化学
医学
新陈代谢
基因
酶
蛋白激酶A
作者
Anouk Charlot,Lucas E. Morel,Anthony Bringolf,Isabelle Georg,Anne‐Laure Charles,Fabienne Goupilleau,Bernard Gény,Joffrey Zoll
出处
期刊:Nutrients
[Multidisciplinary Digital Publishing Institute]
日期:2022-06-29
卷期号:14 (13): 2721-2721
被引量:8
摘要
Background: Medium Chain Fatty Acids (MCFAs) are a dietary supplement that exhibit interesting properties, due to their smaller molecular size. The acute consumption of MCFAs is expected to enhance exercise performance. However, the short-term effects of MCFAs on endurance performance remains poorly understood. The aim of our study is to evaluate the octanoic acid (C8)-rich diet effect on endurance capacity, and to explore their molecular and cellular effects. Methods: C57BL/6J mice were fed with a chow diet (Control group) or an octanoic acid-rich diet (C8 diet) for 6 weeks. Spontaneous activity, submaximal and maximal exercise tests were carried out to characterize the exercise capacities of the mice. Beta-oxidation and mitochondrial biogenesis pathways were explored in skeletal muscle by RT-qPCR, Western Blot (Quadriceps) and histochemical staining (Gastrocnemius). Results: Mice fed with a C8-rich diet presented a higher spontaneous activity (p < 0.05) and endurance capacities (p < 0.05) than the control, but no effect on maximal effort was observed. They also presented changes in the skeletal muscle metabolic phenotype, with a higher number of the oxidative fibers, rich in mitochondria. At the molecular level, the C8-diet induced an AMPK activation (p < 0.05), associated with a significant increase in PGC1a and CS gene expression and protein levels. Conclusion: Our study provided evidence that C8-enrichment as a food supplementation improves endurance capacities and activates mitochondrial biogenesis pathways leading to higher skeletal muscle oxidative capacities.
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