AEROSOL TRANSFER OF BLADDER UROTHELIAL AND SMOOTH MUSCLE CELLS ONTO DEMUCOSALIZED COLONIC SEGMENTS FOR PORCINE BLADDER AUGMENTATION IN VIVO: A 6-WEEK EXPERIMENTAL STUDY

No AccessJournal of UrologyReconstruction1 Oct 2005AEROSOL TRANSFER OF BLADDER UROTHELIAL AND SMOOTH MUSCLE CELLS ONTO DEMUCOSALIZED COLONIC SEGMENTS FOR PORCINE BLADDER AUGMENTATION IN VIVO: A 6-WEEK EXPERIMENTAL STUDY ASHRAF T. HAFEZ, KOUROSH AFSHAR, DARIUS J. BÄGLI, ANDRE BAHORIC, KAREN AITKEN, CHARLES R. SMITH, and ANTOINE E. KHOURY ASHRAF T. HAFEZASHRAF T. HAFEZ More articles by this author , KOUROSH AFSHARKOUROSH AFSHAR More articles by this author , DARIUS J. BÄGLIDARIUS J. BÄGLI More articles by this author , ANDRE BAHORICANDRE BAHORIC More articles by this author , KAREN AITKENKAREN AITKEN More articles by this author , CHARLES R. SMITHCHARLES R. SMITH More articles by this author , and ANTOINE E. KHOURYANTOINE E. KHOURY More articles by this author View All Author Informationhttps://doi.org/10.1097/01.ju.0000177727.56790.98AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract Purpose: In a pilot study we developed a cell transfer technology for populating demucosalized colonic segments with bladder urothelium. This process was achieved through aerosol transfer of a single cell suspension consisting of bladder urothelial cells, smooth muscle cells and fibrin glue onto demucosalized colonic segments. We further evaluate this new concept in a controlled study. Materials and Methods: The study was performed on 20 piglets (20 kg). In all animals 50% of the bladder with excised, and a 10 cm segment of the sigmoid was isolated. Animals were then equally divided into 5 groups of 1) colocystoplasty only, 2) demucosalized colocystoplasty, 3) demucosalized colocystoplasty plus covering of the demucosalized sigmoid with fibrin glue only, 4) aerosol application of fibrin glue with single cell suspension of urothelial cells only to the demucosalized colon, and 5) aerosol application of fibrin glue with urothelial and smooth muscle cells to the demucosalized colon. The 4 corners of the augmented segments were marked with 5-zero polypropylene sutures. Animals were sacrificed 6 weeks later and the surface area of the augmented segment was measured. Segments were submitted to histological and immunohistochemical analysis. Results: The surface area of the augmented segments showed an increase in group 1 animals, stabilization in groups 4 and 5, and marked reduction in groups 2 and 3. On hematoxylin and eosin, and Masson trichrome staining all group 1 animals showed normal colonic epithelium of the augment. All animals in groups 2 and 3 showed excessive scarring with urothelial coverage only at the augment periphery, while the central augment area showed no epithelium. Segments from groups 4 and 5 showed confluent epithelial covering with no fibrosis. There was no evidence of colonic epithelial re-growth in any animal in groups 2 to 5. Cytokeratin 7 and uroplakin III staining demonstrated complete coverage of the augmented segment with urothelium only in groups 4 and 5. Conclusions: The addition of aerosolized cells of urological origin is a viable augmentation approach that appears to achieve the much sought after inhibition of intrinsic fibrosis and contraction of colonic segments when incorporated into the urinary tract without this cellular component. Moreover, this technique appears to provide a histologically normal, confluent urothelium, which sets the stage for prevention of the well-documented biochemical aberrations inherent in augments containing gastrointestinal epithelium. 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Link, Google Scholar 20 : Collagen directly stimulates bladder smooth muscle cell growth in vitro: regulation by extracellular regulated mitogen activated protein kinase. J Urol2003; 170: 2072. Link, Google Scholar From The Urology & Nephrology Center, Mansoura University, Mansoura, Egypt (ATH), Divisions of Urology (KA, DJB, AEK), Surgical Research (AB, KA) and Pathology (CRS), Hospital for Sick Children, University of Toronto, Toronto, Ontario, Canada© 2005 by American Urological Association, Inc.FiguresReferencesRelatedDetails Volume 174Issue 4 Part 2October 2005Page: 1663-1668 Advertisement Copyright & Permissions© 2005 by American Urological Association, Inc.KeywordsbladderfibrinurotheliumMetricsAuthor Information ASHRAF T. HAFEZ More articles by this author KOUROSH AFSHAR More articles by this author DARIUS J. BÄGLI More articles by this author ANDRE BAHORIC More articles by this author KAREN AITKEN More articles by this author CHARLES R. SMITH More articles by this author ANTOINE E. KHOURY More articles by this author Expand All Advertisement PDF downloadLoading ...