医学
免疫系统
CD8型
肿瘤微环境
细胞毒性T细胞
活检
CD80
癌症
内科学
外科肿瘤学
川地68
胃肠病学
肿瘤科
免疫组织化学
免疫学
化学
体外
生物化学
CD40
作者
Alessia D’Ignazio,Paweł Kabata,Maria Raffaella Ambrosio,Karol Połom,Luigi Marano,Luigi Giusto Spagnoli,Alessandra Ongaro,Linda Pieretti,Daniele Marrelli,Ivano Biviano,Franco Roviello
标识
DOI:10.1016/j.clnesp.2020.05.012
摘要
Background & aims This study is focused on the impact of enteral immunonutrition on the cell-mediated immune response in the microenvironment of gastric and colorectal cancers. Methods This is a prospective pilot study approved by the local Ethics Committee. The immunophenotypic structure of the immune cells before (on the biopsy) and after (on the surgical sample) the administration of the immunonutrition in 16 patients is compared with 8 patients receiving regular diet. The samples of non-tumour tissue from sleeve-gastrectomy are used as non-neoplastic control. Antibodies were tested: CD4, CD8, PD-1, FOX-P3, CD68, CD163, CD80, CD21, CD56, PD-L1. We applied already well-known scoring systems for the evaluation of the immunohistochemistry and compared our data in the different groups by statistical analysis. Results In treated patients, we detected a modulation of the immune response with higher number of cytotoxic and helper T-lymphocytes in the tumour microenvironment of the surgical specimens compared to the pre-operative biopsy, and a lower number of lymphocytes presenting an exhausted (i.e. double positive CD8 and PD-1 lymphocytes) and regulatory (i.e. double positive CD4 and FOX-P3 lymphocytes) phenotype. Moreover we observed the M1 polarization with a lower number of CD163 positive macrophages and the inhibition of the PD-1/PD-L1 pathway in treated patients. Conclusions The immunonutrition impacts on the tumoral microenvironment of gastric and colorectal cancer activating the inflammatory pathway, in terms of humoral and cellular response.
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