Notch信号通路
移植
少突胶质细胞
免疫染色
奥利格2
髓鞘
医学
神经科学
细胞生物学
免疫学
病理
生物
信号转导
中枢神经系统
内科学
免疫组织化学
作者
Hao Yuan,Chang‐Le Fang,Yanping Deng,Jin Huang,Rui‐Ze Niu,Jilin Chen,Ting‐Bao Chen,Zhijun Zhu,Li Chen,Ting‐Hua Wang,Liu‐Lin Xiong
出处
期刊:Neurochirurgie
[Elsevier]
日期:2022-02-01
卷期号:68 (2): 188-195
被引量:5
标识
DOI:10.1016/j.neuchi.2021.09.004
摘要
Oligodendrocyte precursor cells (OPCs) are myelinated glial cells of the central nervous system (CNS), able to regenerate oligodendrocytes and myelin. This study aimed to elucidate the effect of A2B5-positive (A2B5+) OPC transplantation in rats with spinal cord contusion (SCC) and to investigate changes in expression of various factors involved in the Notch signaling pathway after OPC transplantation.OPCs were obtained from induced pluripotent stem cells (iPSCs) originating from mouse embryo fibroblasts (MEFs). After identification of iPSCs and iPSC-derived OPCs, A2B5+ OPCs were transplanted into the injured site of rats with SCC one week after SCC insult. Behavioral tests evaluated motor and sensory function 7 days after OPC transplantation. Real-time quantitative polymerase chain reaction (RT-qPCR) determined the expression of various cytokines related to the Notch signaling pathway after OPC transplantation.IPSC-derived OPCs were successfully generated from MEFs, as indicated by positive immunostaining of A2B5, PDGFα and NG2. Further differentiation of OPCs was identified by immunostaining of Olig2, Sox10, Nkx2.2, O4, MBP and GFAP. Importantly, myelin formation was significantly enhanced in the SCC+ OPC group and SCI-induced motor and sensory dysfunction was largely alleviated by A2B5+ OPC transplantation. Expression of factors involved in the Notch signaling pathway (Notch-1, Numb, SHARP1 and NEDD4) was significantly increased after OPC transplantation.A2B5+ OPC transplantation attenuates motor and sensory dysfunction in SCC rats by promoting myelin formation, which may be associated with change in expression of factors involved in the Notch signaling pathway.
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