脂质体
PEG比率
纳米载体
色谱法
化学
药品
药理学
医学
生物化学
财务
经济
作者
Wenjing Tang,Zui Zhang,Cheng Li,Yuxiu Chu,Jun Qian,Tianlei Ying,Weiyue Lu,Changyou Zhan
出处
期刊:Nano Letters
[American Chemical Society]
日期:2021-11-23
卷期号:21 (23): 10107-10113
被引量:13
标识
DOI:10.1021/acs.nanolett.1c03946
摘要
PEGylated nanocarriers have gained increasing attention due to reduced toxicity and enhanced circulation compared with free drugs. According to guidances of drug regulatory departments worldwide, it is crucial to determine free and liposomal drug concentrations; however, the conventional used separation methods including dialysis, ultrafiltration, and solid-phase extraction (SPE) have drawbacks of time-consuming, drug leakage, environmental pollution or error bias of trace level drug. Here we developed a facile PEG-scFv-based separation method combined with HPLC to quantify free doxorubicin (DOX) and liposomal DOX in plasma. Anti-PEG single chain variable fragment antibody (PEG-scFv) was adopted to sediment PEGylated liposomes by simple incubation and low speed centrifugation. Compared to SPE, it demonstrated sufficient accuracy and sensitivity to evaluate free and liposomal DOX with intact liposomes. Therefore, it can serve as an alternative approach of SPE, which is suitable for quality assessment and pharmacokinetics evaluation of PEGylated liposomal drugs and possible other PEGylated nanocarriers.
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