亚硫酸盐
胱硫醚β合酶
同型半胱氨酸
亚硫酸氢钠
化学
亚硫酸钠
亚硫酸氢盐
生物化学
促炎细胞因子
蛋氨酸
防腐剂
半胱氨酸
细胞内
炎症
酶
钠
内科学
食品科学
基因表达
医学
有机化学
DNA甲基化
氨基酸
基因
作者
Wei Wu,Gaoqing Fu,Rongrong Xuan,Leilei Zhai,Yujia Lu,Min Tang,Jingwangwei Liu,Chundan Zhang,Haimin Chen,Feng Wang
标识
DOI:10.1016/j.toxlet.2022.01.019
摘要
Sulfites and other preservatives are considered food additives to prevent pathogen growth in food, and they are generally regarded as safe since the late 1950s. However, the possible effects of sulfites on potential damage to host intestinal tissue remain largely unexplored. Given that endogenous sulfite mainly comes from the metabolism of biothiol, we attempted to clarify the relationship among biothiol levels, gut and food additives sulfite, including sodium bisulfite (NaHSO3), and the possible mechanism of sulfite affecting the intestine. In the present study, the NaHSO3 treatments markedly increased the homocysteine (Hcy) level but decreased the cysteine (Cys) level by promoting the expression of Hcy synthase and inhibiting the activities of cystathionine β-synthase and cystathionine γ-lyase in NCM460 cells. The level of methionine (Met) was not significantly changed, but NaHSO3 promoted ROS-mediated NF-κB signaling pathway, and increased the expressions of proinflammatory cytokines by regulating the levels of Hcy and Cys in NCM460 cells. Vitamin B6 (VB6) supplementation successfully ameliorated NaHSO3-induced damage in NCM460 cells and the colon of Balb/c mice. Altogether, our study provided valuable insights into the safety evaluation of food preservatives. Besides, VB6 could be used as a promising candidate in novel therapies for sodium bisulfite-induced intestinal inflammation.
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