自噬
衰老
活性氧
细胞生物学
线粒体
TFEB
氧化应激
化学
生物
生物化学
细胞凋亡
作者
Meijun Guo,Ke Zhang,Duoduo Zhang,Y. Zhou,Linyuan Liu,Yu-Wei Wu,Xingtao Zhou,Shaoping Nie
标识
DOI:10.1016/j.jfutfo.2022.06.005
摘要
Polysaccharides from genus Ganoderma have prominent anti-aging effect, but their mechanisms are incompletely clarified. In our previous experiments, Ganoderma atrum polysaccharide (PSG) was exhibited to significantly alleviate senescence and DNA damage of A375 cells. To investigate its underlying mechanism, we conducted RNA-Seq analysis and found that PSG upregulated autophagy and mitochondria pathways compared to the model group. Further experiments showed that PSG relieved mitochondrial dysfunction via inhibiting the accumulation of reactive oxygen species (ROS) and loss of the mitochondrial membrane potential (MMP). Meanwhile, PSG activated autophagy and enhanced lysosomal function through transcription factor EB (TFEB). Interestingly, the pretreatment of autophagy inhibitors bafilomycin A1 (Baf A1) reversed the ROS decline induced by PSG, which subsequently increased cellular senescence of A375. In addition, PSG had a similar anti-aging effect on normal fibroblast WI-38. Taken together, PSG might reduce ROS levels through activating autophagy, which in turn suppressed mitochondrial dysfunction and cellular senescence.
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