胶质母细胞瘤
星形胶质细胞
免疫细胞化学
星形细胞瘤
病理
胶质瘤
脑瘤
细胞培养
癌症研究
原代培养
胶质瘤
中枢神经系统
医学
生物
神经科学
化学
遗传学
作者
Mansoureh Hashemi,Rafieh Alizadeh
出处
期刊:Bio-protocol
[Bio-Protocol]
日期:2017-04-20
卷期号:7 (8)
被引量:2
标识
DOI:10.21769/bioprotoc.2241
摘要
Glioblastoma multiforme (GBM) is a grade 4 astrocytoma tumor in central nervous system. Astrocytes can be isolated from human GBM. Study of astrocytes can provide insights about the formation, progression and recurrence of glioblastoma. For using isolated astrocytes, new studies can be designed in the fields of pharmacology, neuroscience and neurosurgery for glioblastoma treatment. This protocol describes the details for preparing high purity primary astrocytes from human GBM. Tumor tissue is disrupted using mechanical dissociation and chemical digestion in this protocol. 2 weeks after plating the cell suspension in culture, primary astrocytes are available for further subculturing and immunocytochemistry of S100-beta antigen.
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