交链孢酚
真菌毒素
化学
共轭体系
结合
玉米赤霉烯酮
乙醚
色谱法
食品科学
有机化学
数学
数学分析
聚合物
作者
A. A. Hildebrand,Beate N. Kohn,Erika Pfeiffer,Daniel Wefers,Manfred Metzler,Mirko Bunzel
标识
DOI:10.1021/acs.jafc.5b00806
摘要
The mycotoxins alternariol (AOH) and alternariol-9-O-methyl ether (AME) carry three and two phenolic hydroxyl groups, respectively, which makes them candidates for the formation of conjugated metabolites in plants. Such conjugates may escape routine methods of analysis and have therefore been termed masked or, more recently, modified mycotoxins. We report now that AOH and AME are extensively conjugated in suspension cultures of tobacco BY-2 cells. Five conjugates of AOH were identified by MS and NMR spectroscopy as β-d-glucopyranosides (attached in AOH 3- or 9-position) as well as their 6′-malonyl derivatives, and as a gentiobiose conjugate. For AME, conjugation resulted in the d-glucopyranoside (mostly attached in the AME 3-position) and its 6′- and 4′-malonyl derivatives. Pronounced differences were noted for the quantitative pattern of AOH and AME conjugates as well as for their phytotoxicity. Our in vitro study demonstrates for the first time that masked mycotoxins of AOH and AME can be formed in plant cells.
科研通智能强力驱动
Strongly Powered by AbleSci AI