Design Glycosyltransferases with High Glycosyl Transfer Efficiency to Efficiently Produce Isoquercetin from Quercetin

) exhibited a 103-fold higher activity than wild-type MiCGT. To enable cost-effective production, sucrose synthase GmSUS was coupled with PCAA to regenerate UDP-glucose during quercetin glycosylation, yielding 3.91 mM isoquercetin with a 78.1% conversion rate. This work overcomes a major bottleneck in isoquercetin biosynthesis and offers a practical strategy for its application in the food industry.