阿米卡星
化学计量学
傅里叶变换红外光谱
化学
色谱法
抗生素
化学工程
工程类
生物化学
作者
Jie C. Chow,Bontha Venkata Subrahmanya Lokesh,Gabriel Akyirem Akowuah
出处
期刊:Current Analytical Chemistry
[Bentham Science]
日期:2024-02-06
卷期号:20
标识
DOI:10.2174/0115734110278516240129174949
摘要
Background: Amikacin belongs to the class of aminoglycoside antibiotics used in the treatment of gram-negative bacterial infections. It is resistant to the aminoglycosides modifying enzymes, making it a clinically effective drug in multidrug-resistant infections. Method: In this study, a simple Attenuated Total Reflectance-Fourier Transform Infrared Spectroscopy was used for the quantification of amikacin in amikacin sulphate injection. The infrared spectra were generated in the spectral range of 4000–667 cm-1. The calibration curve was computed through TQ Analyst Pro edition software, and the partial least square regression analysis found the linearity in the range of 10-60% w/w. objective: This study described a simple and non-destructive Attenuated Total Reflectance Fourier Transform Infrared Spectroscopy for the determination of amikacin in amikacin sulphate for injection. Results: The best calibration results were obtained in the spectral region from 1040 to 1020 cm-1 with a correlation coefficient (r2) of 1.000. The residual mean standard error (RMSEC) value was 0.00235. The percent relative standard deviation (%RSD) values for intra-day and inter-day precision were less than 8.0. The percent relative error (%RE) values were calculated and found in between the range of 0.52 to 5.60. The percent recovery of the amikacin estimation was 113.09 ± 4.27(n=3). method: The pure amikacin was diluted using pure and dried potassium bromide to make serial concentrations of 1-100 %w/w. All samples were scanned between the wavenumber range of 4000-667cm-1 using ATR-FTIR spectroscopy. All the spectra were processed using TQ Analyst Pro Software using the PLS algorithm for obtaining a calibration curve. The specific spectral bands were taken in the finger-print region especially 1500-667 cm-1. The best calibration was showed between 1040-1020cm-1. Conclusion: This validated method is considered a green method, which is suitable for the routine analysis of amikacin in amikacin sulphate injections. conclusion: This calibrated and validated green method is suitable for the routine analysis of amikacin in amikacin sulphate injection and other pharmaceutical formulations.
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