封堵器
血脑屏障
埃文斯蓝
缺血
医学
再灌注损伤
紧密连接
生物杀虫素A
病理
药理学
化学
内分泌学
内科学
中枢神经系统
染料木素
生物化学
大豆黄酮
作者
LONGSHENG FU,JINFANG HU,Feng Shao,YAOQI WU,Wei Bai,MINGJIN JIANG,Hao Chen,LIHUA CHEN,YANNI LV
出处
期刊:Biocell
日期:2023-01-01
卷期号:47 (8): 1869-1883
被引量:1
标识
DOI:10.32604/biocell.2023.028602
摘要
Background: Biochanin A is an excellent dietary isoflavone that has the concomitant function of both medicine and foodstuff. The attenuation function of biochanin A on blood-brain barrier (BBB) damage induced by cerebral ischemia-reperfusion remains unclear. Methods: C57BL/6 mice were subjected to 1 h middle cerebral artery occlusion (MCAO) followed by 24 h reperfusion. The infarct volume of the brain was stained by TTC, while leakage of the brain was quantitatively stained by Evans blue, and the neurologic deficit score was measured. Microglial-induced morphologic changes were observed via immunofluorescence staining, and rolling and adhering leukocytes in venules were observed via two-photon imaging, while the inner fluorescein isothiocyanate-albumin of venules were compared with those of surrounding interstitial area through venular albumin leakage. Results: The attenuation effect of biochanin A on tight junction injury was compared in ischemia-reperfusion mice or conventional knockdown of leucine-rich α2-glycoprotein 1 (Lrg1) mice. Biochanin A could ameliorate BBB injury in mice with cerebral ischemia-reperfusion in a dose-dependent manner by strengthening the immunostaining volume of occludin, claudin-5, and zonula occludens-1. The amoeba morphologic changes of microglial combined with the elevated expression of Lrg1 could be relieved under the treatment of biochanin A. Biochanin A played a countervailing role on the rolling leukocytes in the vessel, while the leakage of blood vessels was reduced. Biochanin A diminished its functions to further improved attenuation for tight junction injury on conventional Lrg1-knockout mice, as well as the inhibition effects on TGF-β1, and the phosphorylation of suppressor of mothers against decapentaplegic 2 (Smad2)/Smad2 via western blot assay. Conclusion: Biochanin A could alleviate tight junction injury induced by cerebral ischemia-reperfusion and blocked the Lrg1/TGF-β/Smad2 pathway to modulate leukocyte migration patterns.
科研通智能强力驱动
Strongly Powered by AbleSci AI