A Multifunctional Tetrahedral DNA Nanostructure for the Simultaneous Sensitive Detection and Imaging of miRNA and Zinc Ions in Living Cells

with detection limits of 0.79 pM and 33.46 nM, respectively, which were much lower than the reported literature. More importantly, LTDNS with a spatial confinement effect and low diffusion coefficient could achieve accurate localization imaging of targets to significantly enhance imaging clarity. The strategy provides an innovative method for simultaneous sensitive detection and accurate imaging of multiple biomarkers and has broad application prospects in early disease diagnosis.