失调
生物
肠道菌群
炎症
结肠炎
乳酸脱氢酶
微生物学
免疫学
生物化学
酶
作者
Savannah Taylor,Maria G. Winter,Caroline C. Gillis,Laice Alves da Silva,Amanda Lee Dobbins,Matthew K. Muramatsu,Angel G. Jimenez,Rachael B. Chanin,Luisella Spiga,Ernesto M. Llano,Vivian Rojas,Jiwoong Kim,Renato Lima Santos,Wenhan Zhu,Sebastian Winter
出处
期刊:Microbiome
[Springer Nature]
日期:2022-11-26
卷期号:10 (1)
被引量:10
标识
DOI:10.1186/s40168-022-01389-7
摘要
Intestinal inflammation disrupts the microbiota composition leading to an expansion of Enterobacteriaceae family members (dysbiosis). Associated with this shift in microbiota composition is a profound change in the metabolic landscape of the intestine. It is unclear how changes in metabolite availability during gut inflammation impact microbial and host physiology.We investigated microbial and host lactate metabolism in murine models of infectious and non-infectious colitis. During inflammation-associated dysbiosis, lactate levels in the gut lumen increased. The disease-associated spike in lactate availability was significantly reduced in mice lacking the lactate dehydrogenase A subunit in intestinal epithelial cells. Commensal E. coli and pathogenic Salmonella, representative Enterobacteriaceae family members, utilized lactate via the respiratory L-lactate dehydrogenase LldD to increase fitness. Furthermore, mice lacking the lactate dehydrogenase A subunit in intestinal epithelial cells exhibited lower levels of inflammation in a model of non-infectious colitis.The release of lactate by intestinal epithelial cells during gut inflammation impacts the metabolism of gut-associated microbial communities. These findings suggest that during intestinal inflammation and dysbiosis, changes in metabolite availability can perpetuate colitis-associated disturbances of microbiota composition. Video Abstract.
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