肌毒素
生物
毒液
遗传学
Crotalus公司
基因
计算生物学
蛇毒
基因组
转录组
进化生物学
基因表达
生态学
作者
Siddharth S. Gopalan,Blair W Perry,Drew R. Schield,Cara F. Smith,Stephen P. Mackessy,Todd A. Castoe
出处
期刊:Toxicon
[Elsevier]
日期:2022-09-01
卷期号:216: 92-106
标识
DOI:10.1016/j.toxicon.2022.06.014
摘要
Crotamine, myotoxin a and homologs are short peptides that often comprise major fractions of rattlesnake venoms and have been extensively studied for their bioactive properties. These toxins are thought to be important for rapidly immobilizing mammalian prey and are implicated in serious, and sometimes fatal, responses to envenomation in humans. While high quality reference genomes for multiple venomous snakes are available, the loci that encode myotoxins have not been successfully assembled in any existing genome assembly. Here, we integrate new and existing genomic and transcriptomic data from the Prairie Rattlesnake ( Crotalus viridis viridis) to reconstruct, characterize, and infer the chromosomal locations of myotoxin-encoding loci. We integrate long-read transcriptomics (Pacific Bioscience's Iso-Seq) and short-read RNA-seq to infer gene sequence diversity and characterize patterns of myotoxin and paralogous β-defensin expression across multiple tissues. We also identify two long non-coding RNA sequences which both encode functional myotoxins, demonstrating a newly discovered source of venom coding sequence diversity. We also integrate long-range mate-pair chromatin contact data and linked-read sequencing to infer the structure and chromosomal locations of the three myotoxin-like loci. Further, we conclude that the venom-associated myotoxin is located on chromosome 1 and is adjacent to non-venom paralogs. Consistent with this locus contributing to venom composition, we find evidence that the promoter of this gene is selectively open in venom gland tissue and contains transcription factor binding sites implicated in broad trans -regulatory pathways that regulate snake venoms. This study provides the best genomic reconstruction of myotoxin loci to date and raises questions about the physiological roles and interplay between myotoxin and related genes, as well as the genomic origins of snake venom variation. • Primary rattlesnake venom myotoxins are found on chromosome 1, next to β-defensins. • Myotoxin regulatory features evolved convergently with other major venom families. • Long non-coding RNA contain regions encoding both myotoxin and other small peptides. • Myotoxin copy number alone does not account for levels of transcript expression.
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