击倒阻力
生物
遗传学
溴氰菊酯
清脆的
黑腹果蝇
等位基因
氯菊酯
基因驱动
Cas9
突变
人口
抗药性
基因敲除
基因
毒理
苯二威
杀虫剂
医学
环境卫生
农学
作者
Bhagyashree Kaduskar,Raja Babu Singh Kushwah,Ankush Auradkar,Annabel Guichard,Menglin Li,Jared B. Bennett,Alison Henrique Ferreira Julio,John M. Marshall,Craig Montell,Ethan Bier
标识
DOI:10.1038/s41467-021-27654-1
摘要
A recurring target-site mutation identified in various pests and disease vectors alters the voltage gated sodium channel (vgsc) gene (often referred to as knockdown resistance or kdr) to confer resistance to commonly used insecticides, pyrethroids and DDT. The ubiquity of kdr mutations poses a major global threat to the continued use of insecticides as a means for vector control. In this study, we generate common kdr mutations in isogenic laboratory Drosophila strains using CRISPR/Cas9 editing. We identify differential sensitivities to permethrin and DDT versus deltamethrin among these mutants as well as contrasting physiological consequences of two different kdr mutations. Importantly, we apply a CRISPR-based allelic-drive to replace a resistant kdr mutation with a susceptible wild-type counterpart in population cages. This successful proof-of-principle opens-up numerous possibilities including targeted reversion of insecticide-resistant populations to a native susceptible state or replacement of malaria transmitting mosquitoes with those bearing naturally occurring parasite resistant alleles.
科研通智能强力驱动
Strongly Powered by AbleSci AI