拉曼散射
DNA
荧光
化学
纳米技术
共振拉曼光谱
多路复用
拉曼光谱
计算生物学
计算机科学
材料科学
生物化学
物理
光学
生物
电信
作者
Karen Faulds,Ljiljana Fruk,D.C. Robson,D. J. Thompson,A. Enright,Wesley H Smith,David Y. Graham
出处
期刊:Faraday Discussions
[The Royal Society of Chemistry]
日期:2006-04-13
卷期号:132: 261-268
被引量:45
摘要
A new approach for the detection of DNA using surface enhance resonance Raman scattering (SERRS) is reported. The majority of existing techniques use fluorescence spectroscopy with advanced probe design to provide information on the identity of specific DNA sequences down to single base resolution. A new approach to the labelling of DNA is discussed which uses Michael addition to couple thiolated DNA to dye labels specifically designed to attach to silver surfaces. When combined with existing strategies for sensitive detection of DNA using commercially available labels, a new class of biomolecular probe known as a SERRS Beacon was produced. The detection techniques of fluorescence and surface enhanced resonance Raman scattering (SERRS) are combined to give a sensitive and selective system for use in the development and creation of novel assays for specifically defined targets. It demonstrates improved potential for multiplexing analysis.
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