细胞滋养层
合胞滋养细胞
细胞生物学
生物
细胞周期
滋养层
细胞融合
细胞
男科
胎盘
胎儿
怀孕
医学
生物化学
遗传学
作者
Hong-Lan Song,Tai-Hang Liu,Yong‐Heng Wang,Fangfang Li,Lingling Ruan,Enoch Appiah Adu‐Gyamfi,Si-Chen Hu,Xuemei Chen,Yubin Ding,Lijuan Fu
出处
期刊:Reproduction
[Bioscientifica]
日期:2021-06-01
卷期号:161 (6): 633-644
被引量:3
摘要
The syncytiotrophoblast, derived from cytotrophoblast fusion, is responsible for maternal-fetal exchanges, secretion of pregnancy-related hormones, and fetal defense against pathogens. Inadequate cytotrophoblast fusion can lead to pregnancy disorders, such as preeclampsia and fetal growth restriction. However, little is known about the mechanism of cytotrophoblast fusion in both physiological and pathological pregnancy conditions. In this study, P57kip2 (P57), a cell cycle-dependent kinase inhibitor that negatively regulates the cell cycle, was found to be up-regulated during the process of syncytialization in both primary trophoblast cells and BeWo cells. Co-immunofluorescence with proliferation markers Ki67 and Cyclin-CDK factors further showed that P57 specifically localizes in the post-mitotic cytotrophoblast subtype of the early pregnancy villi. Overexpression of P57 promoted trophoblast syncytialization by arresting the cell cycle at the G1/G0 phase and inhibiting proliferation. Blocking of the cell cycle through a serum starvation culture resulted in an enhancement of cytotrophoblast fusion and the up-regulation of P57. In both spontaneous cytotrophoblast fusion and forskolin-induced BeWo cell fusion models, an initial up-regulation of P57 was observed followed by a subsequent down-regulation. These findings indicate that proper expression of P57 at cytotrophoblast differentiation nodes plays an important role in trophoblast syncytialization.
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