生物转化
化学
细菌
生物化学
拉伤
细胞色素P450
微生物学
新陈代谢
生物
酶
遗传学
解剖
作者
Qiaoning Wang,Hongdan Wang,Yaru Jiang,Min Lv,Xiaoyan Wang,Lingxin Chen
标识
DOI:10.1016/j.jhazmat.2020.125023
摘要
Sulfamethoxazole (SMX) has attracted much attention due to its high probability of detection in the environment. Marine bacteria Vibrio diabolicus strain L2–2 has been proven to be able to transform SMX. In this study, the potential resistance and biotransformation mechanism of strain L2–2 to SMX, and key genes responses to SMX at environmental concentrations were researched. KEGG pathways were enriched by down-regulated genes including degradation of L-Leucine, L-Isoleucine, and fatty acid metabolism. Resistance mechanism could be concluded as the enhancement of membrane transport, antioxidation, response regulator, repair proteins, and ribosome protection. Biotransformation genes might involve in arylamine N-acetyltransferases (nat), cytochrome c553 (cyc-553) and acyl-CoA synthetase (acs). At the environmental concentration of SMX (0.1–10 μg/L), nat was not be activated, which meant the acetylation of SMX might not occur in the environment; however, cyc-553 was up-regulated under SMX stress of 1 μg/L, which indicated the hydroxylation of SMX could occur in the environment. Besides, the membrane transport and antioxidation of strain L2–2 could be activated under SMX stress of 10 μg/L. The results provided a better understanding of resistance and biotransformation of bacteria to SMX and would support related researches about the impacts of environmental antibiotics.
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