Establishment of the tracing bone marrow reconstituted nude mice model

Objective To establish the green fluorescent protein (GFP) + bone marrow reconstituted nude mice model.Methods Different doses (4,6,8,10 Gy) X-ray were used to irradiate the nude mice.The hemogram was detected separately 1,3,5,7,14 and 21 days after irradiation in order to confirm the proper irradiation dose for bone marrow damage.One-week after irradiation with such dose,allogeneic transplantation with GFP + bone marrow cells (BMCs) (2 × 107 cells/mice) were executed through the caudal vein.The proportion of GFP + donor cells in peripheral blood was tested.White blood cells (WBCs) of peripheral blood and BMCs were compared before and after transplantation.Results After irradiation with 8 Gy,the number of peripheral blood WBCs was (0.1375 ±0.0510) × 109/L,and BMCs were [(31.73 ±2.56)％] of pre-irradiation nude mice.At 28th day after BMCs transplantion,the number of peripheral blood WBCs was (12.46 ± 0.68) × 109/L and recovered to the level pre-irradiation (12.89 ± 1.04) × 109/L (P ＞ 0.05).The proportion of donor GFP + cells was (63.0 ± 0.6) ％ in peripheral blood.Conclusion Eight Gy is the suitable dose for establishment of the bone marrow damage reconstituted nude mice model.Tail vein injection of GFP + BMCs effectively indicated the reconstitution of bone marrow function,which mainly depends on the donor BMCs. Key words: Irradiation; Bone marrow; Green fluorescent protein mice