体细胞核移植
生发泡
卵母细胞
生物
卵胞浆内精子注射
细胞生物学
体细胞
克隆(编程)
胚胎
表观遗传学
遗传学
胚胎发生
胚泡
体外受精
基因
计算机科学
程序设计语言
作者
Zhiming Han,Yong Cheng,Cheng-Guang Liang,Keith E. Latham
出处
期刊:Methods in Enzymology
日期:2010-01-01
卷期号:: 171-184
被引量:10
标识
DOI:10.1016/s0076-6879(10)76010-9
摘要
Nuclear transfer methods provide an invaluable means of dissecting the genetic and epigenetic control of development, as well as the interactions between ooplasm and nucleus in the oocyte and early embryo. These procedures also provide novel means of manipulating animal genomes (e.g., through cloning with genetically engineered cells), and also have been applied for clinical purposes to treat infertility. This chapter reviews methods employed for a range of nuclear transfer techniques including germinal vesicle transfer, spindle transfer, intracytoplasmic sperm injection, pronuclear transfer, and somatic cell nuclear transfer.
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