化学
DNA
复式(建筑)
变性(裂变材料)
有孔小珠
单股
生物物理学
组合化学
纳米技术
分子生物学
生物化学
核化学
生物
复合材料
材料科学
作者
Wei-Siang Kao,Hung‐Wen Li
标识
DOI:10.1002/jccs.201700142
摘要
Previous methods to prepare single‐stranded DNA ( ssDNA ) substrates are limited to short DNA lengths and inefficient. We have developed an efficient and rapid method to prepare long ssDNA substrates (up to 4000 nt) based on the denaturation of the bead‐captured DNA substrates, with the individual steps optimized. Immobilization of the targeted DNA substrates on the antibody‐modified beads allows easy separation of the denatured targeted ssDNA strand. This method also allows the recovery of the captured strand, making it possible to obtain two ssDNA strands from the same duplex DNA . Within 20 min, 80 nM of the 200 nt ssDNA strand could be obtained from its duplex DNA.
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