循环肿瘤细胞
明胶
液体活检
结直肠癌
癌症
纳米技术
材料科学
癌症研究
生物标志物
生物医学工程
化学
医学
内科学
转移
生物化学
作者
Qinqin Huang,Xinghuan Wang,Chunhui Yuan,Zhaobo He,Lang Rao,Bo Cai,Bolei Chen,Shu‐Heng Jiang,Zhiqiang Li,Jincao Chen,Wei Liu,Feng Guo,Zheng Ao,Chuang Shi,Xingzhong Zhao
出处
期刊:Theranostics
[Ivyspring International Publisher]
日期:2018-01-01
卷期号:8 (6): 1624-1635
被引量:68
摘要
Background: Circulating tumor cells (CTCs) are a burgeoning topic in cancer biomarker discovery research with minimal invasive blood draws. CTCs can be used as potential biomarkers for disease prognosis, early cancer diagnosis and pharmacodynamics. However, the extremely low abundance of CTCs limits their clinical utility because of technical challenges such as the isolation and subsequent detailed molecular and functional characterization of rare CTCs from patient blood samples. Methods: In this study, we present a novel density gradient centrifugation method employing biodegradable gelatin nanoparticles coated on silicon beads for the isolation, release, and downstream analysis of CTCs from colorectal and breast cancer patients. Results: Using clinical patient/spiked samples, we demonstrate that this method has significant CTC-capture efficiency (>80%) and purity (>85%), high CTC release efficiency (94%) and viability (92.5%). We also demonstrate the unparalleled robustness of our method in downstream CTC analyses such as the detection of PIK3CA mutations. Conclusion: The efficiency and versatility of the multifunctional density microbeads approach provides new opportunities for personalized cancer diagnostics and treatments.
科研通智能强力驱动
Strongly Powered by AbleSci AI