生物
质粒
恶臭假单胞菌
鼠疫耶尔森菌
铜绿假单胞菌
遗传学
细菌
伯克氏菌属
微生物学
克隆(编程)
基因
计算生物学
毒力
计算机科学
程序设计语言
作者
Kyoung-Hee Choi,Jared B. Gaynor,Kimberly G. White,Carolina Martínez López,Catharine M. Bosio,RoxAnn R. Karkhoff-Schweizer,Herbert P. Schweizer
出处
期刊:Nature Methods
[Springer Nature]
日期:2005-05-20
卷期号:2 (6): 443-448
被引量:686
摘要
For many bacteria, cloning and expression systems are either scarce or nonexistent. We constructed several mini-Tn7 vectors and evaluated their potential as broad-range cloning and expression systems. In bacteria with a single chromosome, including Pseudomonas aeruginosa, Pseudomonas putida and Yersinia pestis, and in the presence of a helper plasmid encoding the site-specific transposition pathway, site- and orientation-specific Tn7 insertions occurred at a single attTn7 site downstream of the glmS gene. Burkholderia thailandensis contains two chromosomes, each containing a glmS gene and an attTn7 site. The Tn7 system allows engineering of diverse genetic traits into bacteria, as demonstrated by complementing a biofilm-growth defect of P. aeruginosa, establishing expression systems in P. aeruginosa and P. putida, and 'GFP-tagging' Y. pestis. This system will thus have widespread biomedical and environmental applications, especially in environments where plasmids and antibiotic selection are not feasible, namely in plant and animal models or biofilms.
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