相图
等温滴定量热法
化学
相(物质)
核糖核酸
生物化学
基因
有机化学
作者
Yanxian Lin,Yann Fichou,Jennifer N. Rauch,Xuemei Zhang,Kenneth S. Kosik,Songi Han
出处
期刊:Methods in molecular biology
日期:2022-10-31
卷期号:: 269-284
标识
DOI:10.1007/978-1-0716-2597-2_18
摘要
Protein liquid-liquid phase separation (LLPS) has been associated with biological functions and pathological aggregation. Mapping the phase separation conditions is the first step to identify and quantify the driving forces of LLPS. Here, we describe the protocols to draw the phase diagram of tau-RNA LLPS and use the mapped diagram to guide experimental conditions for LLPS-cell coculturing, electron resonance spectroscopy in particular double electron-electron resonance spectroscopy, crosslinking immunoprecipitation, and isothermal titration calorimetry.
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