生物塑料
降级(电信)
生物降解
色谱法
基质(水族馆)
酯酶
化学
水解
地衣芽孢杆菌
酶
生物化学
有机化学
生物
细菌
计算机科学
枯草芽孢杆菌
生态学
电信
遗传学
作者
Su Hyun Kim,Nara Shin,Ji-Hong Jeon,Jeong–Jun Yoon,Jeong Chan Joo,Hee Taek Kim,Shashi Kant Bhatia,Yung‐Hun Yang
标识
DOI:10.1016/j.ab.2023.115390
摘要
To alleviate environmental problems caused by using conventional plastics, bioplastics have garnered significant interest as alternatives to petroleum-based plastics. Despite possessing better degradability traits compared to traditional plastics, the degradation of bioplastics still demands a longer duration than initially anticipated. This necessitates the utilization of degradation strains or enzymes to enhance degradation efficiency, ensuring timely degradation. In this study, a novel screening method to identify bioplastic degraders faster was suggested to circumvent the time-consuming and laborious characteristics of solid-based plate assays. This liquid-based colorimetric method confirmed the extracellular esterase activity with p-nitrophenyl esters. It eliminated the needs to prepare plastic emulsion plates at the initial screening system, shortening the time for the overall screening process and providing more quantitative data. p-nitrophenyl hexanoate (C6) was considered the best substrate among the various p-nitrophenyl esters as substrates. The screening was performed in liquid-based 96-well plates, resulting in the discovery of a novel strain, Bacillus sp. SH09, with a similarity of 97.4% with Bacillus licheniformis. Furthermore, clear zone assays, degradation investigations, scanning electron microscopy, and gel permeation chromatography were conducted to characterize the biodegradation capabilities of the new strain, the liquid-based approach offered a swift and less labor-intensive option during the initial stages.
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