光学
共焦
显微镜
光传递函数
空间频率
光学切片
共焦显微镜
卷积(计算机科学)
相(物质)
图像分辨率
物理
人工智能
计算机视觉
计算机科学
量子力学
人工神经网络
作者
Guillaume Noetinger,Fabrice Lemoult,Sébastien M. Popoff
出处
期刊:Optics Letters
[The Optical Society]
日期:2024-02-22
卷期号:49 (5): 1177-1177
摘要
Structured illumination enables the tailoring of an imaging device's optical transfer function to enhance resolution. We propose the incorporation of a temporal periodic modulation, specifically a rotating mask, to encode multiple transfer functions in the temporal domain. This approach is demonstrated using a confocal microscope configuration. At each scanning position, a temporal periodic signal is recorded. By filtering around each harmonic of the rotation frequency, multiple images of the same object can be constructed. The image carried by the nth harmonic is a convolution of the object with a phase vortex of topological charge n, similar to the outcome when using a vortex phase plate as an illumination. This enables the collection of chosen high spatial frequencies from the sample, thereby enhancing the spatial resolution of the confocal microscope.
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