Natural flocculant chitosan inhibits short-chain fatty acid production in anaerobic fermentation of waste activated sludge

Chitosan (CTS) serves as an excellent natural flocculant in wastewater purification and sludge conditioning, but its potential impact on anaerobic fermentation of waste-activated sludge is unclear. The current study investigated the role of CTS in short-chain fatty acids (SCFAs) generation via sludge alkaline anaerobic fermentation. The results showed a drastic reduction in SCFA production with CTS, showing a maximum inhibition of 33 % at 6 mg/g of total suspended solids. CTS hindered sludge solubilization through flocculation, and acted as a humus precursor, promoting humus formation, and consequently reduced the amount of available substrates. Further, CTS promoted free ammonia production, posing a challenge to enzymes and cell viability. Additionally, CTS increased the population of Rikenellaceae sp. and weakened the dominance of hydrolyzing and acidifying bacteria. This study deepens the understanding of the potential impact of CTS on anaerobic fermentation and provides a theoretical basis for reducing the risk of polymeric flocculants.