Dengue Virus Capsid Protein Facilitates Genome Compaction and Packaging

核糖核酸 衣壳 生物 基因组 登革热病毒 病毒蛋白 核糖核蛋白 RNA病毒 病毒 病毒学 遗传学 基因
作者
Priscilla Li Shan Boon,Ana S. Martins,Xin Ni Lim,Francisco J. Enguita,Nuno C. Santos,Peter J. Bond,Yue Wan,Ivo C. Martins,Roland G. Huber
出处
期刊:International Journal of Molecular Sciences [Multidisciplinary Digital Publishing Institute]
卷期号:24 (9): 8158-8158 被引量:4
标识
DOI:10.3390/ijms24098158
摘要

Dengue virus (DENV) is a single-stranded (+)-sense RNA virus that infects humans and mosquitoes, posing a significant health risk in tropical and subtropical regions. Mature virions are composed of an icosahedral shell of envelope (E) and membrane (M) proteins circumscribing a lipid bilayer, which in turn contains a complex of the approximately 11 kb genomic RNA with capsid (C) proteins. Whereas the structure of the envelope is clearly defined, the structure of the packaged genome in complex with C proteins remains elusive. Here, we investigated the interactions of C proteins with viral RNA, in solution and inside mature virions, via footprinting and cross-linking experiments. We demonstrated that C protein interaction with DENV genomes saturates at an RNA:C protein ratio below 1:250. Moreover, we also showed that the length of the RNA genome interaction sites varies, in a multimodal distribution, consistent with the C protein binding to each RNA site mostly in singlets or pairs (and, in some instances, higher numbers). We showed that interaction sites are preferentially sites with low base pairing, as previously measured by 2'-acetylation analyzed by primer extension (SHAPE) reactivity indicating structuredness. We found a clear association pattern emerged: RNA-C protein binding sites are strongly associated with long-range RNA-RNA interaction sites, particularly inside virions. This, in turn, explains the need for C protein in viral genome packaging: the protein has a chief role in coordinating these key interactions, promoting proper packaging of viral RNA. Such sites are, thus, highly consequential for viral assembly, and, as such, may be targeted in future drug development strategies against these and related viruses.

科研通智能强力驱动
Strongly Powered by AbleSci AI
科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
整齐的忆彤完成签到,获得积分10
刚刚
2秒前
lignin完成签到,获得积分10
2秒前
Xiao完成签到,获得积分10
2秒前
唯梦发布了新的文献求助10
3秒前
3秒前
3秒前
4秒前
4秒前
6秒前
ding应助唯梦采纳,获得10
6秒前
nano_yan发布了新的文献求助10
7秒前
哔哔鱼完成签到,获得积分10
8秒前
wait发布了新的文献求助10
8秒前
李爱国应助Summeryz920采纳,获得10
8秒前
8秒前
mly完成签到 ,获得积分10
9秒前
qiulong发布了新的文献求助10
9秒前
哔哔鱼发布了新的文献求助10
11秒前
12秒前
大胆无春完成签到,获得积分10
13秒前
14秒前
16秒前
Brief发布了新的文献求助10
17秒前
BBking发布了新的文献求助10
17秒前
拼搏的不评完成签到 ,获得积分10
18秒前
云泥发布了新的文献求助10
20秒前
dilli完成签到 ,获得积分0
22秒前
MS903完成签到 ,获得积分10
22秒前
赘婿应助lala采纳,获得10
24秒前
bellapp完成签到 ,获得积分10
24秒前
25秒前
动人的怀柔完成签到,获得积分10
29秒前
demotlx发布了新的文献求助10
30秒前
just do it完成签到,获得积分10
30秒前
jhb完成签到 ,获得积分10
31秒前
xinC完成签到 ,获得积分10
32秒前
BBking完成签到,获得积分10
36秒前
demotlx完成签到,获得积分10
36秒前
科研通AI5应助朽木采纳,获得10
36秒前
高分求助中
【此为提示信息,请勿应助】请按要求发布求助,避免被关 20000
ISCN 2024 – An International System for Human Cytogenomic Nomenclature (2024) 3000
Continuum Thermodynamics and Material Modelling 2000
Encyclopedia of Geology (2nd Edition) 2000
105th Edition CRC Handbook of Chemistry and Physics 1600
Maneuvering of a Damaged Navy Combatant 650
Mindfulness and Character Strengths: A Practitioner's Guide to MBSP 380
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 物理 生物化学 纳米技术 计算机科学 化学工程 内科学 复合材料 物理化学 电极 遗传学 量子力学 基因 冶金 催化作用
热门帖子
关注 科研通微信公众号,转发送积分 3776768
求助须知:如何正确求助?哪些是违规求助? 3322170
关于积分的说明 10209141
捐赠科研通 3037424
什么是DOI,文献DOI怎么找? 1666679
邀请新用户注册赠送积分活动 797625
科研通“疑难数据库(出版商)”最低求助积分说明 757944