生物
祖细胞
肝细胞
祖细胞
体外
体内
细胞生物学
肝病
癌症研究
乙型肝炎病毒
免疫学
细胞分化
移植
干细胞
病毒
生物化学
医学
遗传学
内科学
基因
作者
Gongbo Fu,Weijian Huang,Min Zeng,Zhou Xu,Hongping Wu,Changcheng Liu,Han Wu,Jun Weng,Hong‐Dan Zhang,Yongchao Cai,Charles Ashton,Min Ding,Dan Tang,Baohua Zhang,Yi Gao,Weifeng Yu,Bo Zhai,Zhiying He,Hongyang Wang,He‐Xin Yan
出处
期刊:Cell Research
[Springer Nature]
日期:2018-10-25
卷期号:29 (1): 8-22
被引量:105
标识
DOI:10.1038/s41422-018-0103-x
摘要
The study of pathophysiological mechanisms in human liver disease has been constrained by the inability to expand primary hepatocytes in vitro while maintaining proliferative capacity and metabolic function. We and others have previously shown that mouse mature hepatocytes can be converted to liver progenitor-like cells in vitro with defined chemical factors. Here we describe a protocol achieving efficient conversion of human primary hepatocytes into liver progenitor-like cells (HepLPCs) through delivery of developmentally relevant cues, including NAD + -dependent deacetylase SIRT1 signaling. These HepLPCs could be expanded significantly during in vitro passage. The expanded cells can readily be converted back into metabolically functional hepatocytes in vitro and upon transplantation in vivo. Under three-dimensional culture conditions, differentiated cells generated from HepLPCs regained the ability to support infection or reactivation of hepatitis B virus (HBV). Our work demonstrates the utility of the conversion between hepatocyte and liver progenitor-like cells for studying HBV biology and antiviral therapies. These findings will facilitate the study of liver diseases and regenerative medicine.
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