脂肪细胞
脂肪生成
细胞生物学
脂肪组织
单元格排序
白色脂肪组织
流式细胞术
间质细胞
前体细胞
生物
化学
细胞
内科学
内分泌学
生物化学
分子生物学
癌症研究
医学
作者
Chris Church,Ryan Berry,Matthew S. Rodeheffer
出处
期刊:Methods in Enzymology
日期:2014-01-01
卷期号:: 31-46
被引量:86
标识
DOI:10.1016/b978-0-12-411619-1.00003-3
摘要
White adipose tissue (WAT) is a heterogeneous tissue composed of lipid-filled adipocytes and several nonadipocyte cell populations, including endothelial, blood, uncharacterized stromal, and adipocyte precursor cells. Although lipid-filled adipocytes account for the majority of WAT volume and mass, nonadipocyte cell populations have critical roles in WAT maintenance, growth, and function. As mature adipocytes are terminally differentiated postmitotic cells, differentiation of adipocyte precursors is required for hyperplastic WAT growth during development and in obesity. In this chapter, we present methods to separate adipocyte precursor cells from other nonadipocyte cell populations within WAT for analysis by flow cytometry or purification by fluorescence-activated cell sorting. Additionally, we provide methods to study the adipogenic capacity of purified adipocyte precursor cells ex vivo.
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