Molecularly imprinted electrochemical sensor based on electrode modified by functionalized carbon nanotube for selective detection of uric acid

In this study, we have developed a novel biomimetic electrochemical sensor sensitized with functionalized carbon nanotubes using a molecularly imprinted film as a recognition element for the rapid detection of uric acid. Using K3[Fe(CN)6] as a probe, uric acid imprinted films on electrodes are characterized by voltammetry measurements and electrochemical impedance spectroscopy. The optimizations of experimental steps are conducted by cyclic voltammetry and differential pulse voltammetry. When the imprinted sensor is immersed in the solution containing a certain concentration of uric acid and incubated for a period of time, the oxidation peak current of K3[Fe(CN)6] decreases with the increase of uric acid concentration. Under optimal conditions, the peak current of K3[Fe(CN)6] has a good linear relationship with uric acid concentration at range from 0.1 μM to 3.3 μM with the detection limit of 0.03 μM. The proposed sensor shows high selectivity for rapid detection of uric acid in human serum samples.