生物
肌发生
肌生成素
MyoD公司
细胞生物学
骨骼肌
小RNA
心肌细胞
基因表达
肌源性调节因子
虹鳟
基因表达调控
转录因子
C2C12型
作者
Prasanthi P. Koganti,Jian Wang,Beth M. Cleveland,Hao Ma,Gregory M. Weber,Jianbo Yao
标识
DOI:10.1016/j.mce.2016.12.014
摘要
17β-Estradiol (E2) is a steroid hormone that negatively affects muscle growth in rainbow trout, but the mechanism associated with this response is not fully understood. To better characterize the effects of E2 on muscle, we identified differentially regulated microRNAs (miRNAs) and muscle atrophy-related transcripts in juvenile rainbow trout exposed to E2. Small RNA-Seq analysis of E2-treated vs. control muscle identified 36 differentially expressed miRNAs including those known to be involved in myogenesis, cell cycle, apoptosis, and cell death. Some important myogenic miRNAs, such as miR-133 and miR-206, are upregulated while others like miR-145 and miR-499, are downregulated. Gene Ontology analysis of the target genes regulated by the miRNAs involved in atrophy and cell cycle indicates that E2 influence leads to expansion of quiescent myogenic precursor cell population to address atrophying mature muscle in rainbow trout during sexual development.
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